Alpha-virus Research using a Generic primer assay

Serial Dilutions a, b, c, d, e, f, g, …

Complete replication of hepatitis C virus in cell culture.

Many points of the hepatitis C virus (HCV) life cycle haven’t been reproduced in cell tradition, which has slowed analysis progress on this essential human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles which are infectious in cell tradition (HCVcc).

Replication of HCVcc was strong, producing almost 10(5) infectious items per milliliter inside 48 hours. Virus particles have been filterable and neutralized with a monoclonal antibody towards the viral glycoprotein E2.

Viral entry was depending on mobile expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by a number of HCV-specific antiviral compounds, suggesting that this in vitro system will help within the seek for improved antivirals.

HK-2: an immortalized proximal tubule epithelial cell line from normal adult human kidney.

Studies assessing mechanisms of proximal tubular cell (PTC) physiology and pathophysiology more and more make the most of cell tradition programs to keep away from the complexity of entire organ/entire animal experiments. However, no well-differentiated PTC line derived from grownup human kidney at the moment exists.

Therefore, the aim of this analysis was to ascertain such a line by transduction with human papilloma virus (HPV 16) E6/E7 genes. A major PTC tradition from regular grownup human renal cortex was uncovered to a recombinant retrovirus containing the HPV 16 E6/E7 genes, leading to a cell line designated HK-2 (human kidney-2) which has grown constantly in serum free media for a couple of 12 months.

HK-2 cell development is epidermal development issue dependent and the cells retain a phenotype indicative of well-differentiated PTCs (optimistic for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha three beta 1 integrin, fibronectin; damaging for issue VIII-related antigen, 6.19 antigen and CALLA endopeptidase).

Furthermore, HK-2 cells retain useful traits of proximal tubular epithelium (Na+ dependent/phlorizin delicate sugar transport; adenylate cyclase responsiveness to parathyroid, however to not antidiuretic, hormone). The E6/E7 genes are current within the HK-2 genome, as decided by PCR. To assess its potential usefulness as a software for finding out harm and restore, HK-2 cells have been uncovered to a poisonous focus of H2O2 +/- iron chelation (deferoxamine) or hydroxyl radical scavenger (Na benzoate) remedy.

EIF4B
MBS8565155-01mL 0.1mL
EUR 345

EIF4B
MBS8565155-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4B
MBS8565155-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4B
MBS8565155-01mLAF610 0.1mL(AF610)
EUR 565

EIF4B
MBS8565155-01mLAF635 0.1mL(AF635)
EUR 565

EIF4B siRNA
20-abx915195
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  • 15 nmol
  • 30 nmol

EIF4B siRNA
20-abx915196
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  • Ask for price
  • 15 nmol
  • 30 nmol

EIF4B Peptide
MBS3230136-01mg 0.1mg
EUR 180

EIF4B Peptide
MBS3230136-5x01mg 5x0.1mg
EUR 730

eIF4B antibody
20R-2665 50 ug
EUR 269
Description: Rabbit polyclonal eIF4B antibody

eIF4B antibody
20R-2156 50 ug
EUR 269
Description: Rabbit polyclonal eIF4B antibody

EIF4B Antibody
32833 100ul
EUR 439

EIF4B Antibody
32833-100ul 100ul
EUR 302.4

EIF4B Antibody
E10-30365 100ul
EUR 225
Description: Available in various conjugation types.

eIF4B Antibody
E18-5408-1 50μg/50μl
EUR 145
Description: Available in various conjugation types.

eIF4B Antibody
E18-5408-2 100μg/100μl
EUR 225
Description: Available in various conjugation types.

EIF4B Antibody
E19-7324 100μg/100μl
EUR 225
Description: Available in various conjugation types.

eIF4B Antibody
DF7324 200ul
EUR 420

eIF4B Antibody
DF7324-100ul 100ul
EUR 280

eIF4B Antibody
DF7324-200ul 200ul
EUR 350

EIF4B Antibody
E95405 100μg
EUR 255
Description: Available in various conjugation types.

EIF4B Antibody
E313376 200ul
EUR 275
Description: Available in various conjugation types.

EIF4B antibody
70R-1418 100 ug
EUR 407
Description: Rabbit polyclonal EIF4B antibody raised against the C terminal of EIF4B

EIF4B antibody
70R-17056 50 ul
EUR 289
Description: Rabbit polyclonal EIF4B antibody

eIF4B antibody
70R-34968 100 ug
EUR 294
Description: Purified Rabbit polyclonal eIF4B antibody

EIF4B antibody
70R-4927 50 ug
EUR 467
Description: Rabbit polyclonal EIF4B antibody raised against the middle region of EIF4B

eIF4B Antibody
AF6700 100ul
EUR 420

eIF4B Antibody
AF6700-100ul 100ul
EUR 280

eIF4B Antibody
AF6700-200ul 200ul
EUR 350

eIF4B Antibody
AF7767 200ul
EUR 540

eIF4B Antibody
AF7767-100ul 100ul
EUR 350

eIF4B Antibody
AF7767-200ul 200ul
EUR 450

eIF4B Antibody
AF7767-50ul 50ul
EUR 250

eIF4B Antibody
AF7768 200ul
EUR 540

eIF4B Antibody
AF7768-100ul 100ul
EUR 350

eIF4B Antibody
AF7768-200ul 200ul
EUR 450

eIF4B Antibody
AF7768-50ul 50ul
EUR 250

eIF4B Antibody
AF5408 200ul
EUR 420

eIF4B Antibody
AF5408-100ul 100ul
EUR 280

eIF4B Antibody
AF5408-200ul 200ul
EUR 350

EIF4B Antibody
1-CSB-PA007554GA01HU
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  • 150ul
  • 50ul
Description: A polyclonal antibody against EIF4B. Recognizes EIF4B from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

EIF4B Antibody
1-CSB-PA007554LA01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF4B. Recognizes EIF4B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

EIF4B Antibody
1-CSB-PA007988
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  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF4B. Recognizes EIF4B from Human, Mouse. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000

EIF4B Antibody
ABD7324 100ug
EUR 325

eIF4B Antibody
ABF5408 100ug
EUR 325

EIF4B Antibody
F54732-0.08ML 0.08 ml
EUR 140.25
Description: EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

EIF4B Antibody
F54732-0.4ML 0.4 ml
EUR 322.15
Description: EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

EIF4B Antibody
F40109-0.08ML 0.08 ml
EUR 140.25
Description: EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F

EIF4B Antibody
F40109-0.4ML 0.4 ml
EUR 322.15
Description: EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F

EIF4B Antibody
F40110-0.08ML 0.08 ml
EUR 140.25
Description: EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. It also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F

EIF4B Antibody
F40110-0.4ML 0.4 ml
EUR 322.15
Description: EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. It also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F

EIF4B Antibody
GWB-F9FDFF 0.1 mg Ask for price

EIF4B Antibody
GWB-MN411F 50ug Ask for price

EIF4B antibody
BF0398 100ug
EUR 355

EIF4B Antibody
MBS7048085-005mg 0.05mg
EUR 190

EIF4B Antibody
MBS7048085-01mg 0.1mg
EUR 270

EIF4B Antibody
MBS7048085-5x01mg 5x0.1mg
EUR 1205

EIF4B Antibody
MBS7119124-005mg 0.05mg
EUR 150

EIF4B Antibody
MBS7119124-01mg 0.1mg
EUR 190

EIF4B Antibody
MBS7119124-5x01mg 5x0.1mg
EUR 845

EIF4B Antibody
MBS9408587-005mL 0.05mL
EUR 300

EIF4B Antibody
MBS9408587-01mL 0.1mL
EUR 390

EIF4B Antibody
MBS9408587-5x01mL 5x0.1mL
EUR 1610

EIF4B Antibody
MBS9200361-008mL 0.08mL
EUR 210

EIF4B Antibody
MBS9200361-04mL 0.4mL
EUR 430

EIF4B Antibody
MBS9200361-5x04mL 5x0.4mL
EUR 1910

EIF4B Antibody
MBS8576634-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4B Antibody
MBS8576634-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4B Antibody
MBS8576634-01mLAF610 0.1mL(AF610)
EUR 565

EIF4B Antibody
MBS8576634-01mLAF635 0.1mL(AF635)
EUR 565

EIF4B Antibody
MBS8576634-02mL 0.2mL
EUR 345

eIF4B Antibody
MBS8512035-005mg 0.05mg
EUR 235

eIF4B Antibody
MBS8512035-01mg 0.1mg
EUR 305

eIF4B Antibody
MBS8512035-01mLAF405M 0.1mL(AF405M)
EUR 465

eIF4B Antibody
MBS8512035-01mLAF546 0.1mL(AF546)
EUR 465

eIF4B Antibody
MBS8512035-01mLAF750 0.1mL(AF750)
EUR 465

EIF4B Antibody
MBS8516758-01mg 0.1mg
EUR 305

EIF4B Antibody
MBS8516758-01mLAF405L 0.1mL(AF405L)
EUR 465

EIF4B Antibody
MBS8516758-01mLAF405S 0.1mL(AF405S)
EUR 465

EIF4B Antibody
MBS8516758-01mLAF610 0.1mL(AF610)
EUR 465

EIF4B Antibody
MBS8516758-01mLAF635 0.1mL(AF635)
EUR 465

EIF4B Antibody
MBS8500340-01mg 0.1mg
EUR 325

EIF4B Antibody
MBS8500340-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4B Antibody
MBS8500340-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4B Antibody
MBS8500340-01mLAF610 0.1mL(AF610)
EUR 565

EIF4B Antibody
MBS8500340-01mLAF635 0.1mL(AF635)
EUR 565

EIF4B Antibody
MBS9601579-01mL 0.1mL
EUR 260

EIF4B Antibody
MBS9601579-02mL 0.2mL
EUR 305

EIF4B Antibody
MBS9601579-5x02mL 5x0.2mL
EUR 1220

eIF4B Antibody
MBS9611055-005mL 0.05mL
EUR 245

eIF4B Antibody
MBS9611055-01mL 0.1mL
EUR 305

eIF4B Antibody
MBS9611055-02mL 0.2mL
EUR 365

eIF4B Antibody
MBS9611055-5x02mL 5x0.2mL
EUR 1495

eIF4B Antibody
MBS9611056-005mL 0.05mL
EUR 245

eIF4B Antibody
MBS9611056-01mL 0.1mL
EUR 305

eIF4B Antibody
MBS9611056-02mL 0.2mL
EUR 365

eIF4B Antibody
MBS9611056-5x02mL 5x0.2mL
EUR 1495

eIF4B Antibody
MBS9613721-01mL 0.1mL
EUR 260

eIF4B Antibody
MBS9613721-02mL 0.2mL
EUR 305

eIF4B Antibody
MBS9613721-5x02mL 5x0.2mL
EUR 1220

EIF4B Antibody
MBS9607961-01mL 0.1mL
EUR 260

EIF4B Antibody
MBS9607961-02mL 0.2mL
EUR 305

EIF4B Antibody
MBS9607961-5x02mL 5x0.2mL
EUR 1220

eIF4B Antibody
MBS9503699-005mL 0.05mL
EUR 285

eIF4B Antibody
MBS9503699-01mL 0.1mL
EUR 385

eIF4B Antibody
MBS9503699-5x01mL 5x0.1mL
EUR 1590

EIF4B Antibody
BF0398-100ul 100ul
EUR 210
Description: WB,IF/ICC,ELISA,FCM

EIF4B Antibody
BF0398-200ul 200ul Ask for price
Description: WB,IF/ICC,ELISA,FCM

EIF4B Antibody
BF0398-50ul 50ul
EUR 150
Description: WB,IF/ICC,ELISA,FCM

eIF4B Antibody
B0641-100ul 100μl
EUR 217
Description: eIF4B Rabbit Polyclonal Antibody

eIF4B Antibody
B0641-50ul 50μl
EUR 143.5
Description: eIF4B Rabbit Polyclonal Antibody

Eif4b Antibody
C42180-100ul 100μl
EUR 217
Description: Eif4b Rabbit Polyclonal Antibody

Eif4b Antibody
C42180-50ul 50μl
EUR 143.5
Description: Eif4b Rabbit Polyclonal Antibody

EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (AP)
MBS6120819-02mL 0.2(mL
EUR 980

EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (AP)
MBS6120819-5x02mL 5x0.2mL
EUR 4250

EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (PE)
MBS6121662-02mL 0.2(mL
EUR 980

EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (PE)
MBS6121662-5x02mL 5x0.2mL
EUR 4250

EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (APC)
MBS6120993-02mL 0.2(mL
EUR 980

EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (APC)
MBS6120993-5x02mL 5x0.2mL
EUR 4250

EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (FITC)
MBS6121341-02mL 0.2(mL
EUR 980

EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (FITC)
MBS6121341-5x02mL 5x0.2mL
EUR 4250

EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (Biotin)
MBS6121167-02mL 0.2(mL
EUR 980

EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (Biotin)
MBS6121167-5x02mL 5x0.2mL
EUR 4250

EIF4B Mouse mAb
E2600265 100ul
EUR 225
Description: Available in various conjugation types.

eIF4B Mouse mAb
E2220705 100ul
EUR 225
Description: Available in various conjugation types.

EIF4B Mouse mAb
E2222619 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

EIF4B Mouse mAb
MBS8550829-01mL 0.1mL
EUR 305

EIF4B Mouse mAb
MBS8550829-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4B Mouse mAb
MBS8550829-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4B Mouse mAb
MBS8550829-01mLAF610 0.1mL(AF610)
EUR 565

EIF4B Mouse mAb
MBS8550829-01mLAF635 0.1mL(AF635)
EUR 565

EIF4B Mouse mAb
MBS8538912-01mL 0.1mL
EUR 305

EIF4B Mouse mAb
MBS8538912-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4B Mouse mAb
MBS8538912-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4B Mouse mAb
MBS8538912-01mLAF610 0.1mL(AF610)
EUR 565

EIF4B Mouse mAb
MBS8538912-01mLAF635 0.1mL(AF635)
EUR 565

eIF4B Rabbit mAb
52752 100ul
EUR 499

EIF4B Rabbit pAb
A13300-100ul 100 ul
EUR 369.6

EIF4B Rabbit pAb
A13300-200ul 200 ul
EUR 550.8

EIF4B Rabbit pAb
A13300-20ul 20 ul
EUR 219.6

EIF4B Rabbit pAb
A13300-50ul 50 ul
EUR 267.6

eIF4B Rabbit mAb
E2R26713 100ul
EUR 255
Description: Available in various conjugation types.

EIF4B Rabbit pAb
E2505405 100ul
EUR 225
Description: Available in various conjugation types.

EIF4B Rabbit pAb
E2513300 100ul
EUR 225
Description: Available in various conjugation types.

EIF4B Rabbit pAb
A5405-100ul 100 ul
EUR 369.6

EIF4B Rabbit pAb
A5405-200ul 200 ul
EUR 550.8

EIF4B Rabbit pAb
A5405-20ul 20 ul
EUR 219.6

EIF4B Rabbit pAb
A5405-50ul 50 ul
EUR 267.6

EIF4B cDNA Clone
MBS1266068-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

EIF4B cDNA Clone
MBS1266068-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

Eif4b Rabbit pAb
E44H11884 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

EIF4B Rabbit pAb
MBS8548923-01mL 0.1mL
EUR 305

EIF4B Rabbit pAb
MBS8548923-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4B Rabbit pAb
MBS8548923-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4B Rabbit pAb
MBS8548923-01mLAF610 0.1mL(AF610)
EUR 565

EIF4B Rabbit pAb
MBS8548923-01mLAF635 0.1mL(AF635)
EUR 565

EIF4B Rabbit pAb
MBS8545564-01mL 0.1mL
EUR 305

Only the previous blocked H2O2 cytotoxicity, reproducing outcomes beforehand obtained with freshly remoted rat proximal tubular segments. In conclusion, an immortalized grownup human PTC line has been established by transduction with HPV 16 E6/E7 genes. It seems to be well-differentiated on the idea of its histochemical, immune cytochemical, and useful traits, and it will possibly reproduce experimental outcomes obtained with freshly remoted PTCs. Thus, this new PTC line may have substantial analysis utility.