Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.

Hypoxia is characterised as inadequate oxygen supply to tissues and cells within the physique and is prevalent in lots of human physiology processes and illnesses.

Thus, it’s a lovely state to experimentally research to know its internal mechanisms in addition to to develop and take a look at therapies towards pathological circumstances associated to hypoxia.

Animal fashions in vivo fail to recapitulate some of the important thing hallmarks of human physiology, which ends up in human cell cultures; nonetheless, they’re susceptible to bias, particularly when pericellular oxygen focus (partial stress) doesn’t respect oxygen dynamics in vivoA search of the present literature on the subject revealed this was the case for a lot of authentic research pertaining to experimental fashions of hypoxia in vitro.

Therefore, on this overview, we current proof mandating for the shut management of oxygen ranges in cell tradition fashions of hypoxia. First, we talk about the fundamental bodily legal guidelines required for understanding the oxygen dynamics in vitro, most notably the restricted diffusion by way of a liquid medium that hampers the oxygenation of cells in standard cultures.

We then summarize up-to-date information of methods that assist standardize the tradition setting in a replicable vogue by growing oxygen supply to the cells and measuring pericellular ranges. We additionally talk about how these instruments could also be utilized to mannequin each fixed and intermittent hypoxia in a physiologically related method, contemplating recognized values of partial stress of tissue normoxia and hypoxia in vivo, in comparison with standard cultures incubated at inflexible oxygen stress.

Attention is given to the potential affect of three-dimensional tissue cultures and hypercapnia administration on these fashions. Finally, we talk about the implications of these ideas for cell cultures, which attempt to emulate tissue normoxia, and conclude that the upkeep of exact oxygen ranges is essential in any cell tradition setting.

Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.
Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.

Funding Peyronie’s illness: funding sources for main analysis literature.

 

The goal was to explain funding sources and publication traits for the first literature on Peyronie’s illness (PD), together with direct business research help. A search of EMBASE, PubMed, Scopus, and Web of Science was carried out to establish articles printed from 2006 to 2018. 

EIF3M Peptide

MBS3233926-5x01mg 5x0.1mg
EUR 730

EIF3M antibody

70R-17053 50 ul
EUR 289
Description: Rabbit polyclonal EIF3M antibody

EIF3M antibody

70R-1252 100 ug
EUR 407
Description: Rabbit polyclonal EIF3M antibody raised against the N terminal of EIF3M

EIF3M Antibody

40052-100ul 100ul
EUR 468

EIF3M Antibody

DF12602 200ul
EUR 420

EIF3M Antibody

DF12602-100ul 100ul
EUR 168
Description: WB

EIF3M Antibody

DF12602-200ul 200ul
EUR 210
Description: WB

EIF3M Antibody

1-CSB-PA007542GA01HU
  • Ask for price
  • Ask for price
  • 50ul
  • 150ul
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF

eIF3m Antibody

CSB-PA741956XA01DLU-02mg 0.2mg Ask for price
Description: Recombinant Drosophila melanogaster (Fruit fly) eIF3m protein

eIF3m Antibody

CSB-PA741956XA01DLU-10mg 10mg Ask for price
Description: Recombinant Drosophila melanogaster (Fruit fly) eIF3m protein

EIF3M Antibody

1-CSB-PA745333DSR1HU
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  • 100ul
  • 50ul
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000

EIF3M Antibody

1-CSB-PA745333LA01HU
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  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

eif3m Antibody

CSB-PA745844XA01DIL-02mg 0.2mg Ask for price
Description: Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) eif3m protein

eif3m Antibody

CSB-PA745844XA01DIL-10mg 10mg Ask for price
Description: Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) eif3m protein

EIF3M Antibody

C42179-100ul 100μl
EUR 217
Description: EIF3M Rabbit Polyclonal Antibody

EIF3M Antibody

C42179-50ul 50μl
EUR 143.5
Description: EIF3M Rabbit Polyclonal Antibody

EIF3M Antibody

CAC08051-100ug 100ug
EUR 314

EIF3M Antibody

CAC08051-50ug 50ug
EUR 199.2

eif3m Antibody

CSB-PA920317XA01SXV-02mg 0.2mg Ask for price
Description: Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) eif3m protein

eif3m Antibody

CSB-PA920317XA01SXV-10mg 10mg Ask for price
Description: Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) eif3m protein

EIF3M Antibody

GWB-MR101B 100ug Ask for price

EIF3M Antibody

E306244 100ug/200ul
EUR 275
Description: Available in various conjugation types.

EIF3M antibody

E39-02714 100ug/100ul
EUR 225
Description: Available in various conjugation types.

EIF3M Antibody

E94426 100μg
EUR 255
Description: Available in various conjugation types.

EIF3M Antibody

MBS7045522-005mL 0.05mL
EUR 190

EIF3M Antibody

MBS7045522-01mL 0.1mL
EUR 270

EIF3M Antibody

MBS7045522-5x01mL 5x0.1mL
EUR 1205

EIF3M Antibody

MBS7052917-005mg 0.05mg
EUR 190

EIF3M Antibody

MBS7052917-01mg 0.1mg
EUR 270

EIF3M Antibody

MBS7052917-5x01mg 5x0.1mg
EUR 1205

EIF3M Antibody

MBS9611354-01mL 0.1mL
EUR 260

EIF3M Antibody

MBS9611354-02mL 0.2mL
EUR 305

EIF3M Antibody

MBS9611354-5x02mL 5x0.2mL
EUR 1220

EIF3M Antibody

MBS8504459-01mg 0.1mg
EUR 325

EIF3M Antibody

MBS8504459-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF3M Antibody

MBS8504459-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF3M Antibody

MBS8504459-01mLAF610 0.1mL(AF610)
EUR 565

EIF3M Antibody

MBS8504459-01mLAF635 0.1mL(AF635)
EUR 565

EIF3M Antibody

MBS8526981-01mg 0.1mg
EUR 345

EIF3M Antibody

MBS8526981-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF3M Antibody

MBS8526981-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF3M Antibody

MBS8526981-01mLAF610 0.1mL(AF610)
EUR 565

EIF3M Antibody

MBS8526981-01mLAF635 0.1mL(AF635)
EUR 565

EIF3M Antibody

MBS9408729-01mL 0.1mL
EUR 420

EIF3M Antibody

MBS9408729-5x01mL 5x0.1mL
EUR 1740

EIF3M Rabbit pAb

A4426 1000μL
EUR 262.15

EIF3M Rabbit pAb

A4426-100ul 100 ul
EUR 369.6

EIF3M Rabbit pAb

A4426-200ul 200 ul
EUR 550.8

EIF3M Rabbit pAb

A4426-20ul 20 ul
EUR 219.6

EIF3M Rabbit pAb

A4426-50ul 50 ul
EUR 267.6

EIF3M cDNA Clone

MBS1275438-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

EIF3M cDNA Clone

MBS1275438-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

EIF3M rabbit pAb

E28PN5390 100μl
EUR 255
Description: Available in various conjugation types.

EIF3M Rabbit pAb

E2504426 100ul
EUR 225
Description: Available in various conjugation types.

EIF3M rabbit pAb

E44H14274 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

EIF3M Rabbit pAb

E45R25504N 50 ul
EUR 297.75

EIF3M Rabbit pAb

MBS8545382-01mL 0.1mL
EUR 305

EIF3M Rabbit pAb

MBS8545382-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF3M Rabbit pAb

MBS8545382-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF3M Rabbit pAb

MBS8545382-01mLAF610 0.1mL(AF610)
EUR 565

EIF3M Rabbit pAb

MBS8545382-01mLAF635 0.1mL(AF635)
EUR 565

EIF3M rabbit pAb

UB-GEN-10062 100 ul
EUR 200

Mouse EIF3M siRNA

20-abx915187
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  • Ask for price
  • 15 nmol
  • 30 nmol

Human EIF3M siRNA

20-abx915188
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

EIF3M siRNA (Mouse)

MBS8234352-15nmol 15nmol
EUR 405

EIF3M siRNA (Mouse)

MBS8234352-30nmol 30nmol
EUR 565

EIF3M siRNA (Mouse)

MBS8234352-5x30nmol 5x30nmol
EUR 2450

EIF3M siRNA (Human)

MBS8229867-15nmol 15nmol
EUR 405

EIF3M siRNA (Human)

MBS8229867-30nmol 30nmol
EUR 565

EIF3M siRNA (Human)

MBS8229867-5x30nmol 5x30nmol
EUR 2450

Human EIF3M Protein

abx650910-100g 100 µg
EUR 475

Human EIF3M Protein

20-abx650910
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  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Human EIF3M Protein

abx650910-10g 10 µg
EUR 200

Human EIF3M Protein

abx650910-50g 50 µg
EUR 337.5

anti- EIF3M antibody

FNab02714 100µg
EUR 606.3
Description: Antibody raised against EIF3M

anti- EIF3M antibody

FNab02715 100µg
EUR 702
Description: Antibody raised against EIF3M

EIF3M cloning plasmid

CSB-CL745333HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the EIF3M gene.

EIF3M Blocking Peptide

33R-6523 100 ug
EUR 119
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of EIF3M antibody, catalog no. 70R-1252

EIF3M Blocking Peptide

DF12602-BP 1mg
EUR 234

EIF3M Mouse Monoclonal

MBS768947-01mg 0.1mg
EUR 355

EIF3M Mouse Monoclonal

MBS768947-5x01mg 5x0.1mg
EUR 1395

EIF3M Blocking Peptide

MBS9621307-1mg 1mg
EUR 380

EIF3M Blocking Peptide

MBS9621307-5x1mg 5x1mg
EUR 1650

EIF3M Polyclonal Antibody

30564 100ul
EUR 439

EIF3M Polyclonal Antibody

30564-100ul 100ul
EUR 302.4

EIF3M Polyclonal Antibody

30564-50ul 50ul
EUR 224.4

EIF3M polyclonal antibody

BS70625 50 ul
EUR 358
Description: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

EIF3M Polyclonal Antibody

E-AB-63035-120uL 120uL
EUR 320
Description: Unconjugated

EIF3M Polyclonal Antibody

E-AB-63035-200uL 200uL
EUR 530
Description: Unconjugated

EIF3M Polyclonal Antibody

E-AB-63035-60uL 60uL
EUR 200
Description: Unconjugated

EIF3M Polyclonal Antibody

E-AB-63035-each each Ask for price
Description: Unconjugated

EIF3M Polyclonal Antibody

MBS129018-002mL 0.02mL
EUR 200

EIF3M Polyclonal Antibody

MBS129018-005mL 0.05mL
EUR 255

EIF3M Polyclonal Antibody

MBS129018-01mL 0.1mL
EUR 345

EIF3M Polyclonal Antibody

MBS129018-02mL 0.2mL
EUR 545

EIF3M Polyclonal Antibody

MBS129018-5x02mL 5x0.2mL
EUR 2265

EIF3M Polyclonal Antibody

MBS2541512-006mL 0.06mL
EUR 190

EIF3M Polyclonal Antibody

MBS2541512-012mL 0.12mL
EUR 265

EIF3M Polyclonal Antibody

MBS2541512-02mL 0.2mL
EUR 415

EIF3M Polyclonal Antibody

MBS2541512-5x02mL 5x0.2mL
EUR 1835

EIF3M Polyclonal Antibody

MBS9433695-005mL 0.05mL
EUR 300

EIF3M Polyclonal Antibody

MBS9433695-01mL 0.1mL
EUR 390

EIF3M Polyclonal Antibody

MBS9433695-5x01mL 5x0.1mL
EUR 1610

EIF3M Polyclonal Antibody

RD83079A-120uL 120μL
EUR 360
Description: This gene encodes a protein that is part of the eurkaryotic translation initiation factor 3 complete (eIF-3) required for protein synthesis. Elevated levels of the encoded protein are present in cancer cell lines. Inactivation of the encoded protein has been shown to interfere with translation of herpes virus mRNAs by preventing the association of mRNAs with the ribosomes. A pseudogene of this gene is located on the X chromosome.

EIF3M Polyclonal Antibody

RD83079A-200uL 200μL
EUR 630
Description: This gene encodes a protein that is part of the eurkaryotic translation initiation factor 3 complete (eIF-3) required for protein synthesis. Elevated levels of the encoded protein are present in cancer cell lines. Inactivation of the encoded protein has been shown to interfere with translation of herpes virus mRNAs by preventing the association of mRNAs with the ribosomes. A pseudogene of this gene is located on the X chromosome.

EIF3M Polyclonal Antibody

RD83079A-60uL 60μL
EUR 204
Description: This gene encodes a protein that is part of the eurkaryotic translation initiation factor 3 complete (eIF-3) required for protein synthesis. Elevated levels of the encoded protein are present in cancer cell lines. Inactivation of the encoded protein has been shown to interfere with translation of herpes virus mRNAs by preventing the association of mRNAs with the ribosomes. A pseudogene of this gene is located on the X chromosome.

Eif3m (NM_145380) Mouse Recombinant Protein

E45M46851M5 20 ug
EUR 1924.54

Mouse Eif3m ELISA KIT

ELI-26899m 96 Tests
EUR 1038

Human EIF3M ELISA KIT

ELI-26105h 96 Tests
EUR 988.8

EIF3M ELISA KIT|Human

EF009348 96 Tests
EUR 826.8

Bovine EIF3M ELISA KIT

ELI-48146b 96 Tests
EUR 1113.6

Chicken EIF3M ELISA KIT

ELI-26584c 96 Tests
EUR 1113.6

Eif3m (untagged) - Rat eukaryotic translation initiation factor 3, subunit M (Eif3m)

RN216513 10 µg Ask for price

EIF3M (untagged)-Human eukaryotic translation initiation factor 3, subunit M (EIF3M)

SC116147 10 µg Ask for price

EIF3M (untagged)-Human eukaryotic translation initiation factor 3, subunit M (EIF3M)

SC320414 10 µg Ask for price

Human EIF3M shRNA Plasmid

20-abx957117
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  • 150 µg
  • 300 µg

Mouse EIF3M shRNA Plasmid

20-abx979349
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  • Ask for price
  • 150 µg
  • 300 µg

Human EIF3M Protein Lysate

MBS8410993-002mg 0.02mg
EUR 365

Human EIF3M Protein Lysate

MBS8410993-5x002mg 5x0.02mg
EUR 1410

EIF3M Antibody, HRP conjugated

1-CSB-PA745333LB01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

EIF3M Antibody, HRP conjugated

MBS7052918-005mg 0.05mg
EUR 190

EIF3M Antibody, HRP conjugated

MBS7052918-01mg 0.1mg
EUR 270

EIF3M Antibody, HRP conjugated

MBS7052918-5x01mg 5x0.1mg
EUR 1205

EIF3M Recombinant Protein (Rat)

RP199373 100 ug Ask for price

AAP48293-100UG - EIF3M Peptide

AAP48293-100UG 100ug
EUR 99

EIF3M Antibody, FITC conjugated

1-CSB-PA745333LC01HU
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  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

EIF3M Antibody, FITC conjugated

MBS7052919-005mg 0.05mg
EUR 190

EIF3M Antibody, FITC conjugated

MBS7052919-01mg 0.1mg
EUR 270

EIF3M Antibody, FITC conjugated

MBS7052919-5x01mg 5x0.1mg
EUR 1205

Recombinant Human EIF3M Protein

E30P67030A 50 ug
EUR 295

OACA08764-50UL - EIF3M Antibody

OACA08764-50UL 50ul
EUR 189

OAAN01144-50UL - EIF3M Antibody

OAAN01144-50UL 50ul
EUR 149

EIF3M Recombinant Protein (Human)

RP010432 100 ug Ask for price

EIF3M Recombinant Protein (Mouse)

RP131303 100 ug Ask for price

PCID1 (EIF3M) (NM_006360) Human Recombinant Protein

E45H39235M5 20 ug
EUR 785.42

EIF3M Rabbit Polyclonal Antibody

BT-AP09564-100ul 100ul Ask for price

EIF3M Rabbit Polyclonal Antibody

BT-AP09564-20ul 20ul Ask for price

EIF3M Rabbit Polyclonal Antibody

BT-AP09564-50ul 50ul Ask for price

EIF3M Antibody, Biotin conjugated

1-CSB-PA745333LD01HU
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  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

EIF3M Rabbit Polyclonal Antibody

JOT-AP09564-100ul 100ul
EUR 220

EIF3M Rabbit Polyclonal Antibody

JOT-AP09564-50ul 50ul
EUR 144

EIF3M Antibody, Biotin conjugated

MBS7052920-005mg 0.05mg
EUR 190

EIF3M Antibody, Biotin conjugated

MBS7052920-01mg 0.1mg
EUR 270

EIF3M Antibody, Biotin conjugated

MBS7052920-5x01mg 5x0.1mg
EUR 1205

OACA08764-100UL - EIF3M Antibody

OACA08764-100UL 100ul
EUR 230

OAAN01144-100UL - EIF3M Antibody

OAAN01144-100UL 100ul
EUR 249

OAAN01144-200UL - EIF3M Antibody

OAAN01144-200UL 200ul
EUR 389

Eif3m (untagged) - Mouse eukaryotic translation initiation factor 3, subunit M (Eif3m), (10ug)

MC207793 10 µg Ask for price

EIF3M (GFP-tagged) - Human eukaryotic translation initiation factor 3, subunit M (EIF3M)

RG205694 10 µg Ask for price

EIF3M Polyclonal Conjugated Antibody

C30564 100ul
EUR 476.4

EIF3M Polyclonal Conjugated Antibody

MBS9441740-01mLAF350 0.1mL(AF350)
EUR 480

EIF3M Polyclonal Conjugated Antibody

MBS9441740-01mLAF405 0.1mL(AF405)
EUR 480

EIF3M Polyclonal Conjugated Antibody

MBS9441740-01mLAF488 0.1mL(AF488)
EUR 480

EIF3M Polyclonal Conjugated Antibody

MBS9441740-01mLAF555 0.1mL(AF555)
EUR 480

EIF3M Polyclonal Conjugated Antibody

MBS9441740-01mLBiotin 0.1mL(Biotin)
EUR 480

Eif3m ORF Vector (Rat) (pORF)

ORF066459 1.0 ug DNA
EUR 607.2

EIF3M ORF Vector (Human) (pORF)

ORF003478 1.0 ug DNA
EUR 114

Eif3m ORF Vector (Mouse) (pORF)

ORF043769 1.0 ug DNA
EUR 607.2

Eif3m (myc-DDK-tagged) - Rat eukaryotic translation initiation factor 3, subunit M (Eif3m)

RR216513 10 µg Ask for price

Eif3m (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 3, subunit M (Eif3m)

MR205798 10 µg Ask for price

EIF3M (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit M (EIF3M)

RC205694 10 µg Ask for price

Human EIF3M Protein Lysate 20ug

IHUEIF3MPLLY20UG each
EUR 213
Description: Human EIF3M Protein Lysate 20ug

PCID1 (EIF3M) (NM_006360) Human Over-expression Lysate

E45H08157-2 each
EUR 395

PCID1 (EIF3M) (NM_006360) Human Over-expression Lysate

LS010480 100ug
EUR 628
Description: Transient overexpression lysate of eukaryotic translation initiation factor 3, subunit M (EIF3M)

EIF3M antibody - N-terminal region

MBS3208963-01mL 0.1mL
EUR 400

EIF3M antibody - N-terminal region

MBS3208963-5x01mL 5x0.1mL
EUR 1760

Articles not printed in English, evaluations, case experiences, editorials, tips, and meta-analysis have been excluded from evaluation. Data collected included yr and journal of publication, main focus, and funding sources. The US Federal Open Payments database was searched for direct business research funds to authors. Four hundred thirty-seven articles met standards. The median quantity of yearly publications was 31.

The most frequent publications have been Journal of Sexual Medicine (25.6%), Urology (8.9%), BJU International (7.3%), and Journal of Urology (5.7%). Plaque excision/grafting was the most typical subject (16.5%) adopted by pathophysiology (15.3%), and intralesional remedy (14.9%). Only 15.1% (n = 66) of articles had a funding supply with solely 2.3% (n = 10) articles receiving any National Institutes of Health (NIH) funding.

In complete, 4.1% (n = 18) of the articles have been business funded, 61.1% (n = 11) of these from Xiaflex producer Endo/Auxilium. Remaining articles appeared self-funded. There have been 1524 distinctive authors and simply 13 obtained any direct business funds, totaling US$718,426. Most PD research is self-funded with solely a small share from NIH or business.

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