Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.

Hypoxia is characterised as inadequate oxygen supply to tissues and cells within the physique and is prevalent in lots of human physiology processes and illnesses.

Thus, it’s a lovely state to experimentally research to know its internal mechanisms in addition to to develop and take a look at therapies towards pathological circumstances associated to hypoxia.

Animal fashions in vivo fail to recapitulate some of the important thing hallmarks of human physiology, which ends up in human cell cultures; nonetheless, they’re susceptible to bias, particularly when pericellular oxygen focus (partial stress) doesn’t respect oxygen dynamics in vivoA search of the present literature on the subject revealed this was the case for a lot of authentic research pertaining to experimental fashions of hypoxia in vitro.

Therefore, on this overview, we current proof mandating for the shut management of oxygen ranges in cell tradition fashions of hypoxia. First, we talk about the fundamental bodily legal guidelines required for understanding the oxygen dynamics in vitro, most notably the restricted diffusion by way of a liquid medium that hampers the oxygenation of cells in standard cultures.

We then summarize up-to-date information of methods that assist standardize the tradition setting in a replicable vogue by growing oxygen supply to the cells and measuring pericellular ranges. We additionally talk about how these instruments could also be utilized to mannequin each fixed and intermittent hypoxia in a physiologically related method, contemplating recognized values of partial stress of tissue normoxia and hypoxia in vivo, in comparison with standard cultures incubated at inflexible oxygen stress.

Attention is given to the potential affect of three-dimensional tissue cultures and hypercapnia administration on these fashions. Finally, we talk about the implications of these ideas for cell cultures, which attempt to emulate tissue normoxia, and conclude that the upkeep of exact oxygen ranges is essential in any cell tradition setting.

Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.
Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.

Funding Peyronie’s illness: funding sources for main analysis literature.

 

The goal was to explain funding sources and publication traits for the first literature on Peyronie’s illness (PD), together with direct business research help. A search of EMBASE, PubMed, Scopus, and Web of Science was carried out to establish articles printed from 2006 to 2018. 

EIF3M Antibody

1-CSB-PA745333DSR1HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000

EIF3M Antibody

1-CSB-PA745333LA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

EIF3M antibody

70R-1252 100 ug
EUR 452.4
Description: Rabbit polyclonal EIF3M antibody raised against the N terminal of EIF3M

EIF3M antibody

70R-17053 50 ul
EUR 522
Description: Rabbit polyclonal EIF3M antibody

EIF3M Antibody

40052-100ul 100ul
EUR 468

EIF3M siRNA

20-abx915187
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

EIF3M siRNA

20-abx915188
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

EIF3M Antibody

R34769-100UG 100 ug
EUR 399

EIF3M Blocking Peptide

DF12602-BP 1mg
EUR 234

EIF3M cloning plasmid

CSB-CL745333HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the EIF3M gene.

anti- EIF3M antibody

FNab02714 100µg
EUR 606.3
Description: Antibody raised against EIF3M

anti- EIF3M antibody

FNab02715 100µg
EUR 702
Description: Antibody raised against EIF3M

EIF3M Polyclonal Antibody

30564-100ul 100ul
EUR 302.4

EIF3M Polyclonal Antibody

30564-50ul 50ul
EUR 224.4

EIF3M Blocking Peptide

33R-6523 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of EIF3M antibody, catalog no. 70R-1252

EIF3M Rabbit pAb

A4426-100ul 100 ul
EUR 369.6

EIF3M Rabbit pAb

A4426-200ul 200 ul
EUR 550.8

EIF3M Rabbit pAb

A4426-20ul 20 ul
EUR 219.6

EIF3M Rabbit pAb

A4426-50ul 50 ul
EUR 267.6

Human EIF3M Protein

20-abx650910
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Anti-EIF3M antibody

PAab02714 100 ug
EUR 426

Anti-EIF3M antibody

STJ23513 100 µl
EUR 332.4
Description: This gene encodes a protein that is part of the eurkaryotic translation initiation factor 3 complete (eIF-3) required for protein synthesis. Elevated levels of the encoded protein are present in cancer cell lines. Inactivation of the encoded protein has been shown to interfere with translation of herpes virus mRNAs by preventing the association of mRNAs with the ribosomes. A pseudogene of this gene is located on the X chromosome.

EIF3M Polyclonal Conjugated Antibody

C30564 100ul
EUR 476.4

Mouse EIF3M shRNA Plasmid

20-abx979349
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Human EIF3M shRNA Plasmid

20-abx957117
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

EIF3M Antibody, HRP conjugated

1-CSB-PA745333LB01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

EIF3M Antibody, FITC conjugated

1-CSB-PA745333LC01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

EIF3M Antibody, Biotin conjugated

1-CSB-PA745333LD01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3M. Recognizes EIF3M from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Bovine EIF3M ELISA KIT

ELI-48146b 96 Tests
EUR 1113.6

Human EIF3M ELISA KIT

ELI-26105h 96 Tests
EUR 988.8

Chicken EIF3M ELISA KIT

ELI-26584c 96 Tests
EUR 1113.6

Mouse Eif3m ELISA KIT

ELI-26899m 96 Tests
EUR 1038

EIF3M ELISA KIT|Human

EF009348 96 Tests
EUR 826.8

EIF3M Recombinant Protein (Mouse)

RP131303 100 ug Ask for price

EIF3M Recombinant Protein (Rat)

RP199373 100 ug Ask for price

EIF3M Recombinant Protein (Human)

RP010432 100 ug Ask for price

Eif3m ORF Vector (Rat) (pORF)

ORF066459 1.0 ug DNA
EUR 607.2

EIF3M ORF Vector (Human) (pORF)

ORF003478 1.0 ug DNA
EUR 114

Eif3m ORF Vector (Mouse) (pORF)

ORF043769 1.0 ug DNA
EUR 607.2

Polyclonal EIF3M / PCID1 Antibody (C-Terminus)

APR11073G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human EIF3M / PCID1 (C-Terminus). This antibody is tested and proven to work in the following applications:

EIF3M sgRNA CRISPR Lentivector set (Human)

K0668901 3 x 1.0 ug
EUR 406.8

Eif3m sgRNA CRISPR Lentivector set (Rat)

K6239701 3 x 1.0 ug
EUR 406.8

Eif3m sgRNA CRISPR Lentivector set (Mouse)

K4087301 3 x 1.0 ug
EUR 406.8

Anti-B5 Receptor / PCID1 / EIF3M antibody

STJ70788 100 µg
EUR 430.8

EIF3M sgRNA CRISPR Lentivector (Human) (Target 1)

K0668902 1.0 ug DNA
EUR 184.8

EIF3M sgRNA CRISPR Lentivector (Human) (Target 2)

K0668903 1.0 ug DNA
EUR 184.8

EIF3M sgRNA CRISPR Lentivector (Human) (Target 3)

K0668904 1.0 ug DNA
EUR 184.8

Eif3m sgRNA CRISPR Lentivector (Rat) (Target 1)

K6239702 1.0 ug DNA
EUR 184.8

Eif3m sgRNA CRISPR Lentivector (Rat) (Target 2)

K6239703 1.0 ug DNA
EUR 184.8

Eif3m sgRNA CRISPR Lentivector (Rat) (Target 3)

K6239704 1.0 ug DNA
EUR 184.8

Eif3m sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4087302 1.0 ug DNA
EUR 184.8

Eif3m sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4087303 1.0 ug DNA
EUR 184.8

Eif3m sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4087304 1.0 ug DNA
EUR 184.8

EIF3M Protein Vector (Human) (pPB-C-His)

PV013909 500 ng
EUR 394.8

EIF3M Protein Vector (Human) (pPB-N-His)

PV013910 500 ng
EUR 394.8

EIF3M Protein Vector (Human) (pPM-C-HA)

PV013911 500 ng
EUR 394.8

EIF3M Protein Vector (Human) (pPM-C-His)

PV013912 500 ng
EUR 394.8

EIF3M Protein Vector (Mouse) (pPB-C-His)

PV175074 500 ng
EUR 723.6

EIF3M Protein Vector (Mouse) (pPB-N-His)

PV175075 500 ng
EUR 723.6

EIF3M Protein Vector (Mouse) (pPM-C-HA)

PV175076 500 ng
EUR 723.6

EIF3M Protein Vector (Mouse) (pPM-C-His)

PV175077 500 ng
EUR 723.6

EIF3M Protein Vector (Rat) (pPB-C-His)

PV265834 500 ng
EUR 723.6

EIF3M Protein Vector (Rat) (pPB-N-His)

PV265835 500 ng
EUR 723.6

EIF3M Protein Vector (Rat) (pPM-C-HA)

PV265836 500 ng
EUR 723.6

EIF3M Protein Vector (Rat) (pPM-C-His)

PV265837 500 ng
EUR 723.6

EIF3M 3'UTR Luciferase Stable Cell Line

TU006751 1.0 ml
EUR 1672.8

Recombinant Eukaryotic Translation Initiation Factor 3M (EIF3M)

4-RPE997Hu01
  • EUR 496.32
  • EUR 256.80
  • EUR 1531.20
  • EUR 590.40
  • EUR 1060.80
  • EUR 408.00
  • EUR 3648.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Eukaryotic Translation Initiation Factor 3M expressed in: E.coli

Eif3m 3'UTR Luciferase Stable Cell Line

TU203891 1.0 ml Ask for price

Eif3m 3'UTR GFP Stable Cell Line

TU253891 1.0 ml Ask for price

Eif3m 3'UTR GFP Stable Cell Line

TU155725 1.0 ml Ask for price

Eif3m 3'UTR Luciferase Stable Cell Line

TU105725 1.0 ml Ask for price

EIF3M 3'UTR GFP Stable Cell Line

TU056751 1.0 ml
EUR 1672.8

Polyclonal Goat Anti-B5 Receptor / PCID1 / EIF3M Antibody

APR11099G 0.1 mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-B5 Receptor / PCID1 / EIF3M . This antibody is tested and proven to work in the following applications:

Recombinant Human EIF3M Protein, His-SUMO, E.coli-100ug

QP7743-ec-100ug 100ug
EUR 489.6

Recombinant Human EIF3M Protein, His-SUMO, E.coli-10ug

QP7743-ec-10ug 10ug
EUR 240

Recombinant Human EIF3M Protein, His-SUMO, E.coli-1mg

QP7743-ec-1mg 1mg
EUR 1958.4

Recombinant Human EIF3M Protein, His-SUMO, E.coli-200ug

QP7743-ec-200ug 200ug
EUR 760.8

Recombinant Human EIF3M Protein, His-SUMO, E.coli-500ug

QP7743-ec-500ug 500ug
EUR 1272

Recombinant Human EIF3M Protein, His-SUMO, E.coli-50ug

QP7743-ec-50ug 50ug
EUR 315.6

Human Eukaryotic translation initiation factor 3 subunit M (EIF3M)

1-CSB-EP745333HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Eukaryotic translation initiation factor 3 subunit M(EIF3M) expressed in E.coli

Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) Antibody

20-abx112378
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) Antibody

20-abx003305
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) Antibody

abx036552-100ug 100 ug
EUR 469.2

Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) Antibody

abx232714-100ug 100 ug
EUR 577.2

Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) Antibody

abx232715-100ug 100 ug
EUR 661.2

Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) Antibody

20-abx322114
  • EUR 526.80
  • EUR 393.60
  • 100 ul
  • 50 ul

Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) Antibody

20-abx318871
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) Antibody (HRP)

20-abx317444
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) Antibody (FITC)

20-abx317445
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) Antibody (Biotin)

20-abx317446
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

EIF3M sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0668905 3 x 1.0 ug
EUR 451.2

Eif3m sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6239705 3 x 1.0 ug
EUR 451.2

Eif3m sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4087305 3 x 1.0 ug
EUR 451.2

Human Eukaryotic Translation Initiation Factor 3 Subunit M (EIF3M) ELISA Kit

abx387102-96tests 96 tests
EUR 1093.2

EIF3M sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K0668906 1.0 ug DNA
EUR 200.4

EIF3M sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K0668907 1.0 ug DNA
EUR 200.4

EIF3M sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K0668908 1.0 ug DNA
EUR 200.4

Eif3m sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K6239706 1.0 ug DNA
EUR 200.4

Eif3m sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K6239707 1.0 ug DNA
EUR 200.4

Eif3m sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K6239708 1.0 ug DNA
EUR 200.4

Eif3m sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4087306 1.0 ug DNA
EUR 200.4

Eif3m sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4087307 1.0 ug DNA
EUR 200.4

Eif3m sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4087308 1.0 ug DNA
EUR 200.4

Articles not printed in English, evaluations, case experiences, editorials, tips, and meta-analysis have been excluded from evaluation. Data collected included yr and journal of publication, main focus, and funding sources. The US Federal Open Payments database was searched for direct business research funds to authors. Four hundred thirty-seven articles met standards. The median quantity of yearly publications was 31.

The most frequent publications have been Journal of Sexual Medicine (25.6%), Urology (8.9%), BJU International (7.3%), and Journal of Urology (5.7%). Plaque excision/grafting was the most typical subject (16.5%) adopted by pathophysiology (15.3%), and intralesional remedy (14.9%). Only 15.1% (n = 66) of articles had a funding supply with solely 2.3% (n = 10) articles receiving any National Institutes of Health (NIH) funding.

In complete, 4.1% (n = 18) of the articles have been business funded, 61.1% (n = 11) of these from Xiaflex producer Endo/Auxilium. Remaining articles appeared self-funded. There have been 1524 distinctive authors and simply 13 obtained any direct business funds, totaling US$718,426. Most PD research is self-funded with solely a small share from NIH or business.

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