Development and characterization of hepatitis C virus genotype 1-7 cell culture systems: role of CD81 and scavenger receptor class B type I and effect of antiviral drugs.

Six main hepatitis C virus (HCV) genotypes and quite a few subtypes have been described, and just lately a seventh main genotype was found. Genotypes present vital molecular and scientific variations, equivalent to differential response to mixture remedy with interferon-alpha and ribavirin.
Recently, HCV analysis has been accelerated by cell tradition techniques primarily based on the distinctive development capability of pressure JFH1 (genotype 2a). By growth of JFH1-based intergenotypic recombinants containing Core, envelope protein 1 and a couple of (E1, E2), p7, and nonstructural protein 2 (NS2) of genotype 6a and 7a strains, in addition to subtype 1b and 2b strains, we have now accomplished a panel of tradition techniques for all main HCV genotypes.
Efficient development in Huh7.5 cells trusted adaptive mutations for HK6a/JFH1 (6a/2a, in E1 and E2) and J4/JFH1 (1b/2a, in NS2 and NS3); viability of J8/JFH1 (2b/2a) and QC69/JFH1 (7a/2a) didn’t require adaptation. To facilitate comparative research, we generated virus shares of genotype 1-7 recombinants with infectivity titers of 10(3.7) to 10(5.2) 50% tissue tradition infectious dose/mL and HCV RNA titers of 10(7.0) to 10(7.9) IU/mL.
Huh7.5 cultures contaminated with genotype 1-6 viruses had comparable unfold kinetics, intracellular Core, NS5A, and lipid quantities, and colocalization of Core and NS5A with lipids. Treatment with interferon-alpha2b however not ribavirin or amantadine confirmed a big antiviral impact.

EIF4EBP1

pro-532 10µg
EUR 60
Description: Recombinant Human Eukaryotic Translation Initiation Factor 4E-Binding Protein 1

eIF4EBP1

MBS8534292-01mL 0.1mL
EUR 345

eIF4EBP1

MBS8534292-01mLAF405L 0.1mL(AF405L)
EUR 565

eIF4EBP1

MBS8534292-01mLAF405S 0.1mL(AF405S)
EUR 565

eIF4EBP1

MBS8534292-01mLAF610 0.1mL(AF610)
EUR 565

eIF4EBP1

MBS8534292-01mLAF635 0.1mL(AF635)
EUR 565

EIF4EBP1 siRNA

20-abx915203
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  • 15 nmol
  • 30 nmol

EIF4EBP1 siRNA

20-abx915204
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  • 15 nmol
  • 30 nmol

EIF4EBP1 siRNA

20-abx901688
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  • 15 nmol
  • 30 nmol

EIF4EBP1 Protein

20-abx260444
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  • 10 ug
  • 1 mg
  • 50 ug

EIF4EBP1 Protein

abx260444-25mg 25 mg
EUR 2337.5

EIF4EBP1 Protein

abx260444-5mg 5 mg
EUR 325

EIF4EBP1 Antibody

35610 100ul
EUR 319

EIF4EBP1 Antibody

35610-100ul 100ul
EUR 302.4

EIF4EBP1 antibody

38227 100ul
EUR 439

EIF4EBP1 antibody

38227-100ul 100ul
EUR 302.4

eIF4EBP1 Antibody

49297 100ul
EUR 499

eIF4EBP1 Antibody

49297-100ul 100ul
EUR 399.6

eIF4EBP1 Antibody

49297-50ul 50ul
EUR 286.8

EIF4EBP1 Antibody

20-abx330243
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  • 100 ug
  • 50 ug

EIF4EBP1 Antibody

E035610 100μg/100μl
EUR 255
Description: Available in various conjugation types.

EIF4EBP1 Antibody

E91248 100ul
EUR 255
Description: Available in various conjugation types.

EIF4EBP1 Antibody

CSB-PA007561KA01HU- each
EUR 402
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

EIF4EBP1 Antibody

CSB-PA007561KA01HU-100ul 100ul
EUR 466.8
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

EIF4EBP1 Antibody

1-CSB-PA007993
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  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

EIF4EBP1 Antibody

1-CSB-PA007994
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  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

EIF4EBP1 Antibody

1-CSB-PA007995
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  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000

EIF4EBP1 Antibody

1-CSB-PA007996
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  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Human. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000

EIF4EBP1 Antibody

1-CSB-PA588410
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  • 100ul
  • 50ul
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100

EIF4EBP1 Antibody

1-CSB-PA623815LA01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200

EIF4EBP1 Antibody

1-CSB-PA087889
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  • 100ul
  • 50ul
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:200-1:1000, IHC:1:50-1:200

EIF4EBP1 Antibody

1-CSB-PA000769
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  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000

EIF4EBP1 Antibody

1-CSB-PA000770
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  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF4EBP1. Recognizes EIF4EBP1 from Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

EIF4EBP1 Antibody

ABD6380 100ug
EUR 325

Eif4ebp1 Antibody

GWB-MS432I 50ug Ask for price

EIF4EBP1 Antibody

abx330243-100g 100 µg Ask for price

EIF4EBP1 Antibody

abx330243-20g 20 µg
EUR 187.5

EIF4EBP1 Antibody

abx330243-50g 50 µg
EUR 250

EIF4EBP1 Antibody

K70031M01A08C-100ug 100 ug
EUR 320
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M01A08C-1mg 1 mg
EUR 1600
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M01A08C-50ug 50 ug
EUR 224
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M02B09C-100ug 100 ug
EUR 320
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M02B09C-1mg 1 mg
EUR 1600
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M02B09C-50ug 50 ug
EUR 224
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M07C11C-100ug 100 ug
EUR 320
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M07C11C-1mg 1 mg
EUR 1600
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M07C11C-50ug 50 ug
EUR 224
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M11E07C-100ug 100 ug
EUR 320
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M11E07C-1mg 1 mg
EUR 1600
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M11E07C-50ug 50 ug
EUR 224
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M12G12C-100ug 100 ug
EUR 320
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M12G12C-1mg 1 mg
EUR 1600
Description: Monoclonal Antibody

EIF4EBP1 Antibody

K70031M12G12C-50ug 50 ug
EUR 224
Description: Monoclonal Antibody

EIF4EBP1 Antibody

MBS7123263-005mL 0.05mL
EUR 190

EIF4EBP1 Antibody

MBS7123263-01mL 0.1mL
EUR 270

EIF4EBP1 Antibody

MBS7123263-5x01mL 5x0.1mL
EUR 1205

EIF4EBP1 Antibody

MBS7123264-005mL 0.05mL
EUR 190

EIF4EBP1 Antibody

MBS7123264-01mL 0.1mL
EUR 270

EIF4EBP1 Antibody

MBS7123264-5x01mL 5x0.1mL
EUR 1205

EIF4EBP1 Antibody

MBS7050277-005mg 0.05mg
EUR 190

EIF4EBP1 Antibody

MBS7050277-01mg 0.1mg
EUR 270

EIF4EBP1 Antibody

MBS7050277-5x01mg 5x0.1mg
EUR 1205

EIF4EBP1 Antibody

MBS7119129-005mg 0.05mg
EUR 150

EIF4EBP1 Antibody

MBS7119129-01mg 0.1mg
EUR 190

EIF4EBP1 Antibody

MBS7119129-5x01mg 5x0.1mg
EUR 845

EIF4EBP1 Antibody

MBS7119130-005mg 0.05mg
EUR 150

EIF4EBP1 Antibody

MBS7119130-01mg 0.1mg
EUR 190

EIF4EBP1 Antibody

MBS7119130-5x01mg 5x0.1mg
EUR 845

EIF4EBP1 Antibody

MBS7119131-005mg 0.05mg
EUR 150

EIF4EBP1 Antibody

MBS7119131-01mg 0.1mg
EUR 190

EIF4EBP1 Antibody

MBS7119131-5x01mg 5x0.1mg
EUR 845

EIF4EBP1 Antibody

MBS7119132-005mg 0.05mg
EUR 150

EIF4EBP1 Antibody

MBS7119132-01mg 0.1mg
EUR 190

EIF4EBP1 Antibody

MBS7119132-5x01mg 5x0.1mg
EUR 845

EIF4EBP1 Antibody

MBS7115878-005mg 0.05mg
EUR 150

EIF4EBP1 Antibody

MBS7115878-01mg 0.1mg
EUR 190

EIF4EBP1 Antibody

MBS7115878-5x01mg 5x0.1mg
EUR 845

EIF4EBP1 Antibody

MBS7115879-005mg 0.05mg
EUR 150

EIF4EBP1 Antibody

MBS7115879-01mg 0.1mg
EUR 190

EIF4EBP1 Antibody

MBS7115879-5x01mg 5x0.1mg
EUR 845

EIF4EBP1 antibody

MBS9413090-005mL 0.05mL
EUR 300

EIF4EBP1 antibody

MBS9413090-01mL 0.1mL
EUR 390

EIF4EBP1 antibody

MBS9413090-5x01mL 5x0.1mL
EUR 1610

EIF4EBP1 Antibody

MBS9404834-01mL 0.1mL
EUR 305

EIF4EBP1 Antibody

MBS9404834-5x01mL 5x0.1mL
EUR 1230

EIF4EBP1 Antibody

MBS9200389-008mL 0.08mL
EUR 210

EIF4EBP1 Antibody

MBS9200389-04mL 0.4mL
EUR 430

EIF4EBP1 Antibody

MBS9200389-5x04mL 5x0.4mL
EUR 1910

EIF4EBP1 Antibody

MBS8500058-01mL 0.1mL
EUR 325

EIF4EBP1 Antibody

MBS8500058-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4EBP1 Antibody

MBS8500058-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4EBP1 Antibody

MBS8500058-01mLAF610 0.1mL(AF610)
EUR 565

EIF4EBP1 Antibody

MBS8500058-01mLAF635 0.1mL(AF635)
EUR 565

EIF4EBP1 Antibody

MBS2526321-006mL 0.06mL
EUR 180

EIF4EBP1 Antibody

MBS2526321-012mL 0.12mL
EUR 260

EIF4EBP1 Antibody

MBS2526321-02mL 0.2mL
EUR 405

EIF4EBP1 Antibody

MBS2526321-5x02mL 5x0.2mL
EUR 1725

eIF4EBP1 Antibody

MBS9436044-005mL 0.05mL
EUR 325

eIF4EBP1 Antibody

MBS9436044-01mL 0.1mL
EUR 435

eIF4EBP1 Antibody

MBS9436044-5x01mL 5x0.1mL
EUR 1810

EIF4EBP1 Antibody

MBS9607037-01mL 0.1mL
EUR 260

EIF4EBP1 Antibody

MBS9607037-02mL 0.2mL
EUR 305

EIF4EBP1 Antibody

MBS9607037-5x02mL 5x0.2mL
EUR 1220

EIF4EBP1 Mouse mAb

E2600238 100ul
EUR 225
Description: Available in various conjugation types.

EIF4EBP1 Mouse mAb

E2600239 100ul
EUR 225
Description: Available in various conjugation types.

EIF4EBP1 siRNA (Rat)

MBS8219782-15nmol 15nmol
EUR 405

EIF4EBP1 siRNA (Rat)

MBS8219782-30nmol 30nmol
EUR 565

EIF4EBP1 siRNA (Rat)

MBS8219782-5x30nmol 5x30nmol
EUR 2450

EIF4EBP1 Mouse mAb

MBS8550810-01mL 0.1mL
EUR 305

EIF4EBP1 Mouse mAb

MBS8550810-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4EBP1 Mouse mAb

MBS8550810-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4EBP1 Mouse mAb

MBS8550810-01mLAF610 0.1mL(AF610)
EUR 565

EIF4EBP1 Mouse mAb

MBS8550810-01mLAF635 0.1mL(AF635)
EUR 565

EIF4EBP1 Mouse mAb

MBS8550811-01mL 0.1mL
EUR 305

EIF4EBP1 Mouse mAb

MBS8550811-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4EBP1 Mouse mAb

MBS8550811-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4EBP1 Mouse mAb

MBS8550811-01mLAF610 0.1mL(AF610)
EUR 565

EIF4EBP1 Mouse mAb

MBS8550811-01mLAF635 0.1mL(AF635)
EUR 565

eIF4EBP1 Rabbit mAb

59211 100ul
EUR 339

eIF4EBP1 Rabbit pAb

E2381568 100ul
EUR 225
Description: Available in various conjugation types.

EIF4EBP1 Rabbit pAb

E2501248 100ul
EUR 225
Description: Available in various conjugation types.

eIF4EBP1 Rabbit mAb

A19045-100ul 100 ul
EUR 492

eIF4EBP1 Rabbit mAb

A19045-200ul 200 ul
EUR 685.2

eIF4EBP1 Rabbit mAb

A19045-20ul 20 ul
EUR 265.2

eIF4EBP1 Rabbit mAb

A19045-50ul 50 ul
EUR 344.4

EIF4EBP1 Rabbit pAb

A16667-100ul 100 ul
EUR 369.6

EIF4EBP1 Rabbit pAb

A16667-200ul 200 ul
EUR 550.8

EIF4EBP1 Rabbit pAb

A16667-20ul 20 ul
EUR 219.6

EIF4EBP1 Rabbit pAb

A16667-50ul 50 ul
EUR 267.6

EIF4EBP1 cDNA Clone

MBS1277863-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

EIF4EBP1 cDNA Clone

MBS1277863-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

eIF4EBP1 Rabbit pAb

MBS9145063-002mL 0.02mL
EUR 200

eIF4EBP1 Rabbit pAb

MBS9145063-005mL 0.05mL
EUR 255

eIF4EBP1 Rabbit pAb

MBS9145063-01mL 0.1mL
EUR 345

eIF4EBP1 Rabbit pAb

MBS9145063-02mL 0.2mL
EUR 545

eIF4EBP1 Rabbit pAb

MBS9145063-5x02mL 5x0.2mL
EUR 2265

eIF4EBP1 Rabbit pAb

MBS9145066-002mL 0.02mL
EUR 220

eIF4EBP1 Rabbit pAb

MBS9145066-005mL 0.05mL
EUR 295

eIF4EBP1 Rabbit pAb

MBS9145066-01mL 0.1mL
EUR 430

eIF4EBP1 Rabbit pAb

MBS9145066-02mL 0.2mL
EUR 600

eIF4EBP1 Rabbit pAb

MBS9145066-5x02mL 5x0.2mL
EUR 2525

EIF4EBP1 Rabbit pAb

MBS8543032-01mL 0.1mL
EUR 305

EIF4EBP1 Rabbit pAb

MBS8543032-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4EBP1 Rabbit pAb

MBS8543032-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4EBP1 Rabbit pAb

MBS8543032-01mLAF610 0.1mL(AF610)
EUR 565

EIF4EBP1 Rabbit pAb

MBS8543032-01mLAF635 0.1mL(AF635)
EUR 565

EIF4EBP1 Rabbit pAb

MBS8544344-01mL 0.1mL
EUR 305

EIF4EBP1 Rabbit pAb

MBS8544344-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF4EBP1 Rabbit pAb

MBS8544344-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF4EBP1 Rabbit pAb

MBS8544344-01mLAF610 0.1mL(AF610)
EUR 565

EIF4EBP1 Rabbit pAb

MBS8544344-01mLAF635 0.1mL(AF635)
EUR 565

eIF4EBP1 Rabbit mAb

MBS4752718-01mL 0.1mL
EUR 450

eIF4EBP1 Rabbit mAb

MBS4752718-5x01mL 5x0.1mL
EUR 1540

eIF4EBP1 Rabbit pAb

A1248 200μL
EUR 623.48

eIF4EBP1 Rabbit pAb

A16667 20μL
EUR 173.58

eIF4EBP1 Rabbit mAb

A19045 500μL
EUR 343.62

eIF4EBP1 Rabbit mAb

A24691 100μL
EUR 138.16

EIF4EBP1 siRNA (Human)

MBS8233322-15nmol 15nmol
EUR 405

EIF4EBP1 siRNA (Human)

MBS8233322-30nmol 30nmol
EUR 565

EIF4EBP1 siRNA (Human)

MBS8233322-5x30nmol 5x30nmol
EUR 2450

EIF4EBP1 siRNA (Mouse)

MBS8212875-15nmol 15nmol
EUR 405

EIF4EBP1 siRNA (Mouse)

MBS8212875-30nmol 30nmol
EUR 565

EIF4EBP1 siRNA (Mouse)

MBS8212875-5x30nmol 5x30nmol
EUR 2450

EIF4EBP1 Antibody (T69)

MBS9206453-008mL 0.08mL
EUR 210

EIF4EBP1 Antibody (T69)

MBS9206453-04mL 0.4mL
EUR 430

EIF4EBP1 Antibody (T69)

MBS9206453-5x04mL 5x0.4mL
EUR 1910

EIF4EBP1 (pT36) Antibody

20-abx123363
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  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

EIF4EBP1 (pT69) Antibody

20-abx123364
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  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

EIF4EBP1 (pT36) Antibody

abx031976-400ul 400 ul
EUR 627.6

EIF4EBP1 (pT36) Antibody

abx031976-80l 80 µl
EUR 343.2
Infection with all genotypes might be blocked by particular antibodies in opposition to the putative coreceptors CD81 and scavenger receptor class B sort I in a dose-dependent method. Finally, neutralizing antibodies in chosen persistent part HCV sera had differential results in opposition to genotype 1-7 viruses.
CONCLUSIONS
We accomplished and characterised a panel of JFH1-based cell tradition techniques of all seven main HCV genotypes and necessary subtypes and used these viruses in comparative research of antivirals, HCV receptor interplay, and neutralizing antibodies.